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mini sub cell gt horizontal electrophoresis system  (Bio-Rad)


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    Bio-Rad mini sub cell gt horizontal electrophoresis system
    Mini Sub Cell Gt Horizontal Electrophoresis System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mini+sub+cell+gt+systems/Mini-Sub+Cell+GT+Horizontal+Electrophoresis+System/pmc13052146-92-0-7
    Average 95 stars, based on 84 article reviews
    mini sub cell gt horizontal electrophoresis system - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Transferring:

    Article Title: High-throughput Preparation of DNA, RNA, and Protein from Cryopreserved Human iPSCs for Multi-omics Analysis
    Article Snippet: AllPrep DNA/RNA/Protein Mini Kit (50) (QIAGEN, cat. no. 80004) QIAshredder Kit (250) (QIAGEN, cat. no. 79656) QIAshredder Mini Spin Column (QIAGEN (in AllPrep kit)) Allprep DNA Mini Spin Column (QIAGEN (in AllPrep kit)) RNeasy Mini Spin Column (QIAGEN (in AllPrep kit)) Buffer RW1 (QIAGEN (in AllPrep kit)) Buffer RLT (QIAGEN (in AllPrep kit)) Buffer RPE (QIAGEN (in AllPrep kit)) Buffer AW1 (QIAGEN (in AllPrep kit)) Buffer AW2 (QIAGEN (in AllPrep kit)) Buffer EB (QIAGEN (in AllPrep kit)) RNaseZap (Invitrogen, cat no. AM9780) Absolute Ethanol (100% ethanol, 200 proof) (Fisher BioReagents, cat. no. BP2818500) 2-Mercaptoethanol, ≥99.0% (Sigma-Aldrich, cat. no. M6250) UltraPure Distilled Water (Invitrogen, cat. no. 10977015) Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (Ham) (Life Technologies, cat. no. 11330032) (store at 4 °C) Trypan Blue Stain 0.4% (ThermoFisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"T10282","term_id":"471631","term_text":"T10282"}} T10282 ) MicroAmp Optical 96-Well Reaction Plate (Life Technologies, cat. no. N8010560) Qubit dsDNA BR Assay Kit (ThermoFisher Scientific, cat. no. {"type":"entrez-protein","attrs":{"text":"Q32853","term_id":"75280860","term_text":"Q32853"}} Q32853 ) (see individual components for storage conditions) Gel Loading Dye, Blue (6X) (New England Biolabs, cat. no. B7021S) UltraPureTM TBE Buffer, 10X (InvitrogenTM, cat. no. 15581044) 1 kb DNA Ladder (New England Biolabs, cat. no. N3232S) (store at −20 °C) UltraPureTM Agarose (InvitrogenTM, cat. no. 16500100) MIDORI Green Advance (NN Genetics, cat. no. MG04) (store at 4 °C) .. Research® Plus Single Channel Pipette, Adjustable 0.1-2.5 μL micropipette (Eppendorf, cat. no. 3123000012) Research® Plus Single Channel Pipette, Adjustable 20-200 μL micropipette (Eppendorf, cat. no. 3123000055) Research® Plus Single Channel Pipette, Adjustable 100-1000 μL micropipette (Eppendorf, cat. no. 3123000063) RPT Filter Tips, 10 μL XL (TipOne, cat. no. 1120-3810) RPT Filter Tips, 200 μL, graduated (TipOne, cat. no. 1120-8810) RPT Filter Tips, 1000 μL, graduated (TipOne, cat. no.1111-2830) 15 mL High-Clarity Polypropylene Conical Tubes (Corning, cat. no. 352096) Safe-Lock Tubes 1.5mL, natural (Eppendorf, cat. no. 022363204) Protein LoBind Tubes 2.0 mL (Eppendorf, cat. no. 022431102) Isotemp Digital Control Water Bath Model 205 (ThermoFisher Scientific, cat. no. 154625Q) Compact Drybath D 100-120V (ThermoFisher Scientific, cat. no. 8887 1002) Sorvall Legend Micro 21 Microcentrifuge (ThermoFisher Scientific, cat no.75002436) Sorvall Legend X1 Centrifuge (ThermoFisher Scientific, cat. no. 75004221) Countess II FL Automated Cell Counter (ThermoFisher Scientific, cat. no. AMQAF1000) Countess Cell Counting Chamber Slides (ThermoFisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"C10283","term_id":"56146369","term_text":"C10283"}} C10283 ) Mini Sub-Cell GT Gel Caster (Bio-Rad, cat. no. 1704422) Mini-Sub Cell GT UV-Transparent Gel Tray, 7 x 10 cm (Bio-Rad, cat. no. 1704435) 15-Well Comb for use with Mini-Sub cell GT systems (Bio-Rad, cat. no. 1704465) Wide Mini-Sub Cell GT UV-Transparent Gel Tray, 15 x 7 cm (Bio-Rad, cat. no. 1704426) 30-Well Comb for use with wide Mini-Sub cell GT systems (Bio-Rad, cat. no. 1704449) Mini-Sub Cell GT Cell (Bio-Rad, cat. no. 1704406) Wide Mini-Sub Cell GT Cell (Bio-Rad, cat. no. 1704405) PowerPacTM Basic Power Supply (Bio-Rad, cat. no. 1645050) QuBit 4 Fluorometer (ThermoFisher Scientific, cat. no. {"type":"entrez-protein","attrs":{"text":"Q33238","term_id":"75281074","term_text":"Q33238"}} Q33238 ) GelDoc-ItTM Imaging System 2UV Transilluminator (UVP, cat. no. 361809) .. Preparation [QIAGEN instructions] Prepare RLT buffer by adding 10 μL 2-Mercaptoethanol, ≥99.0% to every 1 mL of RLT used.

    Control:

    Article Title: High-throughput Preparation of DNA, RNA, and Protein from Cryopreserved Human iPSCs for Multi-omics Analysis
    Article Snippet: AllPrep DNA/RNA/Protein Mini Kit (50) (QIAGEN, cat. no. 80004) QIAshredder Kit (250) (QIAGEN, cat. no. 79656) QIAshredder Mini Spin Column (QIAGEN (in AllPrep kit)) Allprep DNA Mini Spin Column (QIAGEN (in AllPrep kit)) RNeasy Mini Spin Column (QIAGEN (in AllPrep kit)) Buffer RW1 (QIAGEN (in AllPrep kit)) Buffer RLT (QIAGEN (in AllPrep kit)) Buffer RPE (QIAGEN (in AllPrep kit)) Buffer AW1 (QIAGEN (in AllPrep kit)) Buffer AW2 (QIAGEN (in AllPrep kit)) Buffer EB (QIAGEN (in AllPrep kit)) RNaseZap (Invitrogen, cat no. AM9780) Absolute Ethanol (100% ethanol, 200 proof) (Fisher BioReagents, cat. no. BP2818500) 2-Mercaptoethanol, ≥99.0% (Sigma-Aldrich, cat. no. M6250) UltraPure Distilled Water (Invitrogen, cat. no. 10977015) Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (Ham) (Life Technologies, cat. no. 11330032) (store at 4 °C) Trypan Blue Stain 0.4% (ThermoFisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"T10282","term_id":"471631","term_text":"T10282"}} T10282 ) MicroAmp Optical 96-Well Reaction Plate (Life Technologies, cat. no. N8010560) Qubit dsDNA BR Assay Kit (ThermoFisher Scientific, cat. no. {"type":"entrez-protein","attrs":{"text":"Q32853","term_id":"75280860","term_text":"Q32853"}} Q32853 ) (see individual components for storage conditions) Gel Loading Dye, Blue (6X) (New England Biolabs, cat. no. B7021S) UltraPureTM TBE Buffer, 10X (InvitrogenTM, cat. no. 15581044) 1 kb DNA Ladder (New England Biolabs, cat. no. N3232S) (store at −20 °C) UltraPureTM Agarose (InvitrogenTM, cat. no. 16500100) MIDORI Green Advance (NN Genetics, cat. no. MG04) (store at 4 °C) .. Research® Plus Single Channel Pipette, Adjustable 0.1-2.5 μL micropipette (Eppendorf, cat. no. 3123000012) Research® Plus Single Channel Pipette, Adjustable 20-200 μL micropipette (Eppendorf, cat. no. 3123000055) Research® Plus Single Channel Pipette, Adjustable 100-1000 μL micropipette (Eppendorf, cat. no. 3123000063) RPT Filter Tips, 10 μL XL (TipOne, cat. no. 1120-3810) RPT Filter Tips, 200 μL, graduated (TipOne, cat. no. 1120-8810) RPT Filter Tips, 1000 μL, graduated (TipOne, cat. no.1111-2830) 15 mL High-Clarity Polypropylene Conical Tubes (Corning, cat. no. 352096) Safe-Lock Tubes 1.5mL, natural (Eppendorf, cat. no. 022363204) Protein LoBind Tubes 2.0 mL (Eppendorf, cat. no. 022431102) Isotemp Digital Control Water Bath Model 205 (ThermoFisher Scientific, cat. no. 154625Q) Compact Drybath D 100-120V (ThermoFisher Scientific, cat. no. 8887 1002) Sorvall Legend Micro 21 Microcentrifuge (ThermoFisher Scientific, cat no.75002436) Sorvall Legend X1 Centrifuge (ThermoFisher Scientific, cat. no. 75004221) Countess II FL Automated Cell Counter (ThermoFisher Scientific, cat. no. AMQAF1000) Countess Cell Counting Chamber Slides (ThermoFisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"C10283","term_id":"56146369","term_text":"C10283"}} C10283 ) Mini Sub-Cell GT Gel Caster (Bio-Rad, cat. no. 1704422) Mini-Sub Cell GT UV-Transparent Gel Tray, 7 x 10 cm (Bio-Rad, cat. no. 1704435) 15-Well Comb for use with Mini-Sub cell GT systems (Bio-Rad, cat. no. 1704465) Wide Mini-Sub Cell GT UV-Transparent Gel Tray, 15 x 7 cm (Bio-Rad, cat. no. 1704426) 30-Well Comb for use with wide Mini-Sub cell GT systems (Bio-Rad, cat. no. 1704449) Mini-Sub Cell GT Cell (Bio-Rad, cat. no. 1704406) Wide Mini-Sub Cell GT Cell (Bio-Rad, cat. no. 1704405) PowerPacTM Basic Power Supply (Bio-Rad, cat. no. 1645050) QuBit 4 Fluorometer (ThermoFisher Scientific, cat. no. {"type":"entrez-protein","attrs":{"text":"Q33238","term_id":"75281074","term_text":"Q33238"}} Q33238 ) GelDoc-ItTM Imaging System 2UV Transilluminator (UVP, cat. no. 361809) .. Preparation [QIAGEN instructions] Prepare RLT buffer by adding 10 μL 2-Mercaptoethanol, ≥99.0% to every 1 mL of RLT used.

    Article Title: 1S Supplementary Information SP94-Targeted Triblock Co-Polymer Nanoparticle Delivers Thymidine Kinase-p53-Nitroreductase Triple Therapeutic Gene and Restores Anticancer Function against Hepatocellular Carcinoma in vivo
    Article Snippet: .. As per the aforementioned protocol, different N/P ratios (1-7) of polymer/pcDNA complexes were prepared in nuclease free water, and the resultant polyplexes were loaded in 0.7% agarose gel along with the equivalent amount of naked pcDNA as control, and electrophoresed in Mini-Sub® Cell GT Systems at 60 V for 45 min. After the run, the gel was imaged in BioRad Gel DocTM XR+ Gel Documentation system (Bio Rad, Hercules, CA) to further quantify and analyze the extent of DNA encapsulation. ..

    Cell Counting:

    Article Title: High-throughput Preparation of DNA, RNA, and Protein from Cryopreserved Human iPSCs for Multi-omics Analysis
    Article Snippet: AllPrep DNA/RNA/Protein Mini Kit (50) (QIAGEN, cat. no. 80004) QIAshredder Kit (250) (QIAGEN, cat. no. 79656) QIAshredder Mini Spin Column (QIAGEN (in AllPrep kit)) Allprep DNA Mini Spin Column (QIAGEN (in AllPrep kit)) RNeasy Mini Spin Column (QIAGEN (in AllPrep kit)) Buffer RW1 (QIAGEN (in AllPrep kit)) Buffer RLT (QIAGEN (in AllPrep kit)) Buffer RPE (QIAGEN (in AllPrep kit)) Buffer AW1 (QIAGEN (in AllPrep kit)) Buffer AW2 (QIAGEN (in AllPrep kit)) Buffer EB (QIAGEN (in AllPrep kit)) RNaseZap (Invitrogen, cat no. AM9780) Absolute Ethanol (100% ethanol, 200 proof) (Fisher BioReagents, cat. no. BP2818500) 2-Mercaptoethanol, ≥99.0% (Sigma-Aldrich, cat. no. M6250) UltraPure Distilled Water (Invitrogen, cat. no. 10977015) Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (Ham) (Life Technologies, cat. no. 11330032) (store at 4 °C) Trypan Blue Stain 0.4% (ThermoFisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"T10282","term_id":"471631","term_text":"T10282"}} T10282 ) MicroAmp Optical 96-Well Reaction Plate (Life Technologies, cat. no. N8010560) Qubit dsDNA BR Assay Kit (ThermoFisher Scientific, cat. no. {"type":"entrez-protein","attrs":{"text":"Q32853","term_id":"75280860","term_text":"Q32853"}} Q32853 ) (see individual components for storage conditions) Gel Loading Dye, Blue (6X) (New England Biolabs, cat. no. B7021S) UltraPureTM TBE Buffer, 10X (InvitrogenTM, cat. no. 15581044) 1 kb DNA Ladder (New England Biolabs, cat. no. N3232S) (store at −20 °C) UltraPureTM Agarose (InvitrogenTM, cat. no. 16500100) MIDORI Green Advance (NN Genetics, cat. no. MG04) (store at 4 °C) .. Research® Plus Single Channel Pipette, Adjustable 0.1-2.5 μL micropipette (Eppendorf, cat. no. 3123000012) Research® Plus Single Channel Pipette, Adjustable 20-200 μL micropipette (Eppendorf, cat. no. 3123000055) Research® Plus Single Channel Pipette, Adjustable 100-1000 μL micropipette (Eppendorf, cat. no. 3123000063) RPT Filter Tips, 10 μL XL (TipOne, cat. no. 1120-3810) RPT Filter Tips, 200 μL, graduated (TipOne, cat. no. 1120-8810) RPT Filter Tips, 1000 μL, graduated (TipOne, cat. no.1111-2830) 15 mL High-Clarity Polypropylene Conical Tubes (Corning, cat. no. 352096) Safe-Lock Tubes 1.5mL, natural (Eppendorf, cat. no. 022363204) Protein LoBind Tubes 2.0 mL (Eppendorf, cat. no. 022431102) Isotemp Digital Control Water Bath Model 205 (ThermoFisher Scientific, cat. no. 154625Q) Compact Drybath D 100-120V (ThermoFisher Scientific, cat. no. 8887 1002) Sorvall Legend Micro 21 Microcentrifuge (ThermoFisher Scientific, cat no.75002436) Sorvall Legend X1 Centrifuge (ThermoFisher Scientific, cat. no. 75004221) Countess II FL Automated Cell Counter (ThermoFisher Scientific, cat. no. AMQAF1000) Countess Cell Counting Chamber Slides (ThermoFisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"C10283","term_id":"56146369","term_text":"C10283"}} C10283 ) Mini Sub-Cell GT Gel Caster (Bio-Rad, cat. no. 1704422) Mini-Sub Cell GT UV-Transparent Gel Tray, 7 x 10 cm (Bio-Rad, cat. no. 1704435) 15-Well Comb for use with Mini-Sub cell GT systems (Bio-Rad, cat. no. 1704465) Wide Mini-Sub Cell GT UV-Transparent Gel Tray, 15 x 7 cm (Bio-Rad, cat. no. 1704426) 30-Well Comb for use with wide Mini-Sub cell GT systems (Bio-Rad, cat. no. 1704449) Mini-Sub Cell GT Cell (Bio-Rad, cat. no. 1704406) Wide Mini-Sub Cell GT Cell (Bio-Rad, cat. no. 1704405) PowerPacTM Basic Power Supply (Bio-Rad, cat. no. 1645050) QuBit 4 Fluorometer (ThermoFisher Scientific, cat. no. {"type":"entrez-protein","attrs":{"text":"Q33238","term_id":"75281074","term_text":"Q33238"}} Q33238 ) GelDoc-ItTM Imaging System 2UV Transilluminator (UVP, cat. no. 361809) .. Preparation [QIAGEN instructions] Prepare RLT buffer by adding 10 μL 2-Mercaptoethanol, ≥99.0% to every 1 mL of RLT used.

    Imaging:

    Article Title: High-throughput Preparation of DNA, RNA, and Protein from Cryopreserved Human iPSCs for Multi-omics Analysis
    Article Snippet: AllPrep DNA/RNA/Protein Mini Kit (50) (QIAGEN, cat. no. 80004) QIAshredder Kit (250) (QIAGEN, cat. no. 79656) QIAshredder Mini Spin Column (QIAGEN (in AllPrep kit)) Allprep DNA Mini Spin Column (QIAGEN (in AllPrep kit)) RNeasy Mini Spin Column (QIAGEN (in AllPrep kit)) Buffer RW1 (QIAGEN (in AllPrep kit)) Buffer RLT (QIAGEN (in AllPrep kit)) Buffer RPE (QIAGEN (in AllPrep kit)) Buffer AW1 (QIAGEN (in AllPrep kit)) Buffer AW2 (QIAGEN (in AllPrep kit)) Buffer EB (QIAGEN (in AllPrep kit)) RNaseZap (Invitrogen, cat no. AM9780) Absolute Ethanol (100% ethanol, 200 proof) (Fisher BioReagents, cat. no. BP2818500) 2-Mercaptoethanol, ≥99.0% (Sigma-Aldrich, cat. no. M6250) UltraPure Distilled Water (Invitrogen, cat. no. 10977015) Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (Ham) (Life Technologies, cat. no. 11330032) (store at 4 °C) Trypan Blue Stain 0.4% (ThermoFisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"T10282","term_id":"471631","term_text":"T10282"}} T10282 ) MicroAmp Optical 96-Well Reaction Plate (Life Technologies, cat. no. N8010560) Qubit dsDNA BR Assay Kit (ThermoFisher Scientific, cat. no. {"type":"entrez-protein","attrs":{"text":"Q32853","term_id":"75280860","term_text":"Q32853"}} Q32853 ) (see individual components for storage conditions) Gel Loading Dye, Blue (6X) (New England Biolabs, cat. no. B7021S) UltraPureTM TBE Buffer, 10X (InvitrogenTM, cat. no. 15581044) 1 kb DNA Ladder (New England Biolabs, cat. no. N3232S) (store at −20 °C) UltraPureTM Agarose (InvitrogenTM, cat. no. 16500100) MIDORI Green Advance (NN Genetics, cat. no. MG04) (store at 4 °C) .. Research® Plus Single Channel Pipette, Adjustable 0.1-2.5 μL micropipette (Eppendorf, cat. no. 3123000012) Research® Plus Single Channel Pipette, Adjustable 20-200 μL micropipette (Eppendorf, cat. no. 3123000055) Research® Plus Single Channel Pipette, Adjustable 100-1000 μL micropipette (Eppendorf, cat. no. 3123000063) RPT Filter Tips, 10 μL XL (TipOne, cat. no. 1120-3810) RPT Filter Tips, 200 μL, graduated (TipOne, cat. no. 1120-8810) RPT Filter Tips, 1000 μL, graduated (TipOne, cat. no.1111-2830) 15 mL High-Clarity Polypropylene Conical Tubes (Corning, cat. no. 352096) Safe-Lock Tubes 1.5mL, natural (Eppendorf, cat. no. 022363204) Protein LoBind Tubes 2.0 mL (Eppendorf, cat. no. 022431102) Isotemp Digital Control Water Bath Model 205 (ThermoFisher Scientific, cat. no. 154625Q) Compact Drybath D 100-120V (ThermoFisher Scientific, cat. no. 8887 1002) Sorvall Legend Micro 21 Microcentrifuge (ThermoFisher Scientific, cat no.75002436) Sorvall Legend X1 Centrifuge (ThermoFisher Scientific, cat. no. 75004221) Countess II FL Automated Cell Counter (ThermoFisher Scientific, cat. no. AMQAF1000) Countess Cell Counting Chamber Slides (ThermoFisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"C10283","term_id":"56146369","term_text":"C10283"}} C10283 ) Mini Sub-Cell GT Gel Caster (Bio-Rad, cat. no. 1704422) Mini-Sub Cell GT UV-Transparent Gel Tray, 7 x 10 cm (Bio-Rad, cat. no. 1704435) 15-Well Comb for use with Mini-Sub cell GT systems (Bio-Rad, cat. no. 1704465) Wide Mini-Sub Cell GT UV-Transparent Gel Tray, 15 x 7 cm (Bio-Rad, cat. no. 1704426) 30-Well Comb for use with wide Mini-Sub cell GT systems (Bio-Rad, cat. no. 1704449) Mini-Sub Cell GT Cell (Bio-Rad, cat. no. 1704406) Wide Mini-Sub Cell GT Cell (Bio-Rad, cat. no. 1704405) PowerPacTM Basic Power Supply (Bio-Rad, cat. no. 1645050) QuBit 4 Fluorometer (ThermoFisher Scientific, cat. no. {"type":"entrez-protein","attrs":{"text":"Q33238","term_id":"75281074","term_text":"Q33238"}} Q33238 ) GelDoc-ItTM Imaging System 2UV Transilluminator (UVP, cat. no. 361809) .. Preparation [QIAGEN instructions] Prepare RLT buffer by adding 10 μL 2-Mercaptoethanol, ≥99.0% to every 1 mL of RLT used.

    Polymer:

    Article Title: 1S Supplementary Information SP94-Targeted Triblock Co-Polymer Nanoparticle Delivers Thymidine Kinase-p53-Nitroreductase Triple Therapeutic Gene and Restores Anticancer Function against Hepatocellular Carcinoma in vivo
    Article Snippet: .. As per the aforementioned protocol, different N/P ratios (1-7) of polymer/pcDNA complexes were prepared in nuclease free water, and the resultant polyplexes were loaded in 0.7% agarose gel along with the equivalent amount of naked pcDNA as control, and electrophoresed in Mini-Sub® Cell GT Systems at 60 V for 45 min. After the run, the gel was imaged in BioRad Gel DocTM XR+ Gel Documentation system (Bio Rad, Hercules, CA) to further quantify and analyze the extent of DNA encapsulation. ..

    Agarose Gel Electrophoresis:

    Article Title: 1S Supplementary Information SP94-Targeted Triblock Co-Polymer Nanoparticle Delivers Thymidine Kinase-p53-Nitroreductase Triple Therapeutic Gene and Restores Anticancer Function against Hepatocellular Carcinoma in vivo
    Article Snippet: .. As per the aforementioned protocol, different N/P ratios (1-7) of polymer/pcDNA complexes were prepared in nuclease free water, and the resultant polyplexes were loaded in 0.7% agarose gel along with the equivalent amount of naked pcDNA as control, and electrophoresed in Mini-Sub® Cell GT Systems at 60 V for 45 min. After the run, the gel was imaged in BioRad Gel DocTM XR+ Gel Documentation system (Bio Rad, Hercules, CA) to further quantify and analyze the extent of DNA encapsulation. ..

    Encapsulation:

    Article Title: 1S Supplementary Information SP94-Targeted Triblock Co-Polymer Nanoparticle Delivers Thymidine Kinase-p53-Nitroreductase Triple Therapeutic Gene and Restores Anticancer Function against Hepatocellular Carcinoma in vivo
    Article Snippet: .. As per the aforementioned protocol, different N/P ratios (1-7) of polymer/pcDNA complexes were prepared in nuclease free water, and the resultant polyplexes were loaded in 0.7% agarose gel along with the equivalent amount of naked pcDNA as control, and electrophoresed in Mini-Sub® Cell GT Systems at 60 V for 45 min. After the run, the gel was imaged in BioRad Gel DocTM XR+ Gel Documentation system (Bio Rad, Hercules, CA) to further quantify and analyze the extent of DNA encapsulation. ..



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